Regulatory

Part:BBa_K2287003:Design

Designed by: Ruiling Cai   Group: iGEM17_UCAS   (2017-10-19)


astCp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a basic part containing a nitrogen-sensitive promoter glnHp2 and its regulatory sequences. The pre-attached RBS frees users from tedious DNA assembly. The riboJ is added to insulate the transcriptional elements, so that they will not interfere with each other. This enables users to test the function of the promoter more precisely.

Source

The DNA of glnHp2 is synthesized according to documented sequences. RiboJ and RBS comes from Lou lab.

References

1. Kiupakis, Alexandros K., and Larry Reitzer. "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli." Journal of bacteriology 184.11 (2002): 2940-2950.


2. Zong, Yeqing, et al. "Insulated transcriptional elements enable precise design of genetic circuits." Nature Communications 8 (2017).